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- Title
Two-photon optogenetics of dendritic spines and neural circuits.
- Authors
Packer, Adam M; Peterka, Darcy S; Hirtz, Jan J; Prakash, Rohit; Deisseroth, Karl; Yuste, Rafael
- Abstract
We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.
- Publication
Nature methods, 2012, Vol 9, Issue 12, p1202
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.2249