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- Title
Robust 4C-seq data analysis to screen for regulatory DNA interactions.
- Authors
van de Werken, Harmen J G; Landan, Gilad; Holwerda, Sjoerd J B; Hoichman, Michael; Klous, Petra; Chachik, Ran; Splinter, Erik; Valdes-Quezada, Christian; Oz, Yuva; Bouwman, Britta A M; Verstegen, Marjon J A M; de Wit, Elzo; Tanay, Amos; de Laat, Wouter
- Abstract
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation.
- Publication
Nature methods, 2012, Vol 9, Issue 10, p969
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.2173