We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Scanning angle interference microscopy reveals cell dynamics at the nanoscale.
- Authors
Paszek, Matthew J; DuFort, Christopher C; Rubashkin, Matthew G; Davidson, Michael W; Thorn, Kurt S; Liphardt, Jan T; Weaver, Valerie M
- Abstract
Emerging questions in cell biology necessitate nanoscale imaging in live cells. Here we present scanning angle interference microscopy, which is capable of localizing fluorescent objects with nanoscale precision along the optical axis in motile cellular structures. We use this approach to resolve nanotopographical features of the cell membrane and cytoskeleton as well as the temporal evolution, three-dimensional architecture and nanoscale dynamics of focal adhesion complexes.
- Publication
Nature methods, 2012, Vol 9, Issue 8, p825
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.2077