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- Title
M-Track: detecting short-lived protein-protein interactions in vivo.
- Authors
Zuzuarregui, Aurora; Kupka, Thomas; Bhatt, Bhumika; Dohnal, Ilse; Mudrak, Ingrid; Friedmann, Christina; Schüchner, Stefan; Frohner, Ingrid E; Ammerer, Gustav; Ogris, Egon
- Abstract
We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.
- Publication
Nature methods, 2012, Vol 9, Issue 6, p594
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.2017