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- Title
Automated whole-cell patch-clamp electrophysiology of neurons in vivo.
- Authors
Kodandaramaiah, Suhasa B; Franzesi, Giovanni Talei; Chow, Brian Y; Boyden, Edward S; Forest, Craig R
- Abstract
Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.
- Publication
Nature methods, 2012, Vol 9, Issue 6, p585
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1993