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- Title
Systematic identification of gene annotation errors in the widely used yeast mutation collections.
- Authors
Ben-Shitrit, Taly; Yosef, Nir; Shemesh, Keren; Sharan, Roded; Ruppin, Eytan; Kupiec, Martin
- Abstract
The baker's yeast mutation collections are extensively used genetic resources that are the basis for many genome-wide screens and new technologies. Anecdotal evidence has previously pointed to the putative existence of a neighboring gene effect (NGE) in these collections. NGE occurs when the phenotype of a strain carrying a particular perturbed gene is due to the lack of proper function of its adjacent gene. Here we performed a large-scale study of NGEs, presenting a network-based algorithm for detecting NGEs and validating software predictions using complementation experiments. We applied our approach to four datasets uncovering a similar magnitude of NGE in each (7-15%). These results have important consequences for systems biology, as the mutation collections are extensively used in almost every aspect of the field, from genetic network analysis to functional gene annotation.
- Publication
Nature methods, 2012, Vol 9, Issue 4, p373
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1890