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- Title
Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine.
- Authors
Song, Chun-Xiao; Clark, Tyson A; Lu, Xing-Yu; Kislyuk, Andrey; Dai, Qing; Turner, Stephen W; He, Chuan; Korlach, Jonas
- Abstract
We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with high confidence.
- Publication
Nature Methods, 2012, Vol 9, Issue 1, p75
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth.1779