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- Title
High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases.
- Authors
Fuqiang Chen; Pruett-Miller, Shondra M.; Yuping Huang; Gjoka, Monika; Duda, Katarzyna; Taunton, Jack; Collingwood, Trevor N.; Frodin, Morten; Davis, Gregory D.
- Abstract
Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mutation, (ii) targeted genomic deletion of up to 100 kb and (iii) targeted insertion of small genetic elements concomitant with large genomic deletions.
- Publication
Nature Methods, 2011, Vol 8, Issue 9, p753
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth.1653