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- Title
A public genome-scale lentiviral expression library of human ORFs.
- Authors
Xiaoping Yang; Boeh, Jesse S.; Xinping Yang; Kourosh Salehi-Ashtiani; Tong Hao; Yun Shen; Rakela Lubonja; Thomas, Sapana R.; Ozan Alkan; Tashfeen Bhimdi; Green, Thomas M.; Johannessen, Cory M.; Silver, Serena J.; Cindy Nguyen; Murray, Ryan R.; Hieronymus, Haley; Balcha, Dawit; Fan, Changyu; Lin, Chenwei; Ghamsari, Lila
- Abstract
Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
- Publication
Nature Methods, 2011, Vol 8, Issue 8, p659
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth.1638