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- Title
Sharper low-power STED nanoscopy by time gating.
- Authors
Vicidomini, Giuseppe; Moneron, Gael; Han, Kyu Y; Westphal, Volker; Ta, Haisen; Reuss, Matthias; Engelhardt, Johann; Eggeling, Christian; Hell, Stefan W
- Abstract
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.
- Publication
Nature methods, 2011, Vol 8, Issue 7, p571
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1624