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- Title
A more efficient method to generate integration-free human iPS cells.
- Authors
Okita, Keisuke; Matsumura, Yasuko; Sato, Yoshiko; Okada, Aki; Morizane, Asuka; Okamoto, Satoshi; Hong, Hyenjong; Nakagawa, Masato; Tanabe, Koji; Tezuka, Ken-ichi; Shibata, Toshiyuki; Kunisada, Takahiro; Takahashi, Masayo; Takahashi, Jun; Saji, Hiroh; Yamanaka, Shinya
- Abstract
We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.
- Publication
Nature methods, 2011, Vol 8, Issue 5, p409
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1591