We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
- Authors
Conrad, Christian; Wünsche, Annelie; Tan, Tze Heng; Bulkescher, Jutta; Sieckmann, Frank; Verissimo, Fatima; Edelstein, Arthur; Walter, Thomas; Liebel, Urban; Pepperkok, Rainer; Ellenberg, Jan
- Abstract
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.
- Publication
Nature methods, 2011, Vol 8, Issue 3, p246
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1558