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- Title
RNAi screening for fat regulatory genes with SRS microscopy.
- Authors
Wang, Meng C; Min, Wei; Freudiger, Christian W; Ruvkun, Gary; Xie, X Sunney
- Abstract
Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.
- Publication
Nature methods, 2011, Vol 8, Issue 2, p135
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1556