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Title
Chemical synthesis of the mouse mitochondrial genome.
Authors
Gibson, Daniel G; Smith, Hamilton O; Hutchison, Clyde A, 3rd; Venter, J Craig; Merryman, Chuck
Abstract
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.