We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A human MAP kinase interactome.
- Authors
Bandyopadhyay, Sourav; Chiang, Chih-yuan; Srivastava, Jyoti; Gersten, Merril; White, Suhaila; Bell, Russell; Kurschner, Cornelia; Martin, Christopher H; Smoot, Mike; Sahasrabudhe, Sudhir; Barber, Diane L; Chanda, Sumit K; Ideker, Trey
- Abstract
Mitogen-activated protein kinase (MAPK) pathways form the backbone of signal transduction in the mammalian cell. Here we applied a systematic experimental and computational approach to map 2,269 interactions between human MAPK-related proteins and other cellular machinery and to assemble these data into functional modules. Multiple lines of evidence including conservation with yeast supported a core network of 641 interactions. Using small interfering RNA knockdowns, we observed that approximately one-third of MAPK-interacting proteins modulated MAPK-mediated signaling. We uncovered the Na-H exchanger NHE1 as a potential MAPK scaffold, found links between HSP90 chaperones and MAPK pathways and identified MUC12 as the human analog to the yeast signaling mucin Msb2. This study makes available a large resource of MAPK interactions and clone libraries, and it illustrates a methodology for probing signaling networks based on functional refinement of experimentally derived protein-interaction maps.
- Publication
Nature methods, 2010, Vol 7, Issue 10, p801
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1506