We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Amplification-free digital gene expression profiling from minute cell quantities.
- Authors
Ozsolak, Fatih; Ting, David T; Wittner, Ben S; Brannigan, Brian W; Paul, Suchismita; Bardeesy, Nabeel; Ramaswamy, Sridhar; Milos, Patrice M; Haber, Daniel A
- Abstract
Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.
- Publication
Nature methods, 2010, Vol 7, Issue 8, p619
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1480