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- Title
Exploring the sequence determinants of amyloid structure using position-specific scoring matrices.
- Authors
Maurer-Stroh, Sebastian; Debulpaep, Maja; Kuemmerer, Nico; Lopez de la Paz, Manuela; Martins, Ivo Cristiano; Reumers, Joke; Morris, Kyle L; Copland, Alastair; Serpell, Louise; Serrano, Luis; Schymkowitz, Joost W H; Rousseau, Frederic
- Abstract
Protein aggregation results in beta-sheet-like assemblies that adopt either a variety of amorphous morphologies or ordered amyloid-like structures. These differences in structure also reflect biological differences; amyloid and amorphous beta-sheet aggregates have different chaperone affinities, accumulate in different cellular locations and are degraded by different mechanisms. Further, amyloid function depends entirely on a high intrinsic degree of order. Here we experimentally explored the sequence space of amyloid hexapeptides and used the derived data to build Waltz, a web-based tool that uses a position-specific scoring matrix to determine amyloid-forming sequences. Waltz allows users to identify and better distinguish between amyloid sequences and amorphous beta-sheet aggregates and allowed us to identify amyloid-forming regions in functional amyloids.
- Publication
Nature methods, 2010, Vol 7, Issue 3, p237
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1432