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- Title
FRT-seq: amplification-free, strand-specific transcriptome sequencing.
- Authors
Mamanova, Lira; Andrews, Robert M; James, Keith D; Sheridan, Elizabeth M; Ellis, Peter D; Langford, Cordelia F; Ost, Tobias W B; Collins, John E; Turner, Daniel J
- Abstract
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
- Publication
Nature methods, 2010, Vol 7, Issue 2, p130
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1417