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- Title
Virtual terminator nucleotides for next-generation DNA sequencing.
- Authors
Jayson Bowers; Mitchell, Judith; Beer, Eric; Buzby, Philip R.; Causey, Marie; Efcavitch, J. William; Jarosz, Mirna; Krzymanska-Olejnik, Edyta; Li Kung; Lipson, Doron; Lowman, Geoffrey M.; Marappan, Subramanian; McInerney, Peter; Platt, Adam; Roy, Atanu; Siddiqi, Suhaib M.; Steinmann, Kathleen; Thompson, John F.
- Abstract
We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
- Publication
Nature Methods, 2009, Vol 6, Issue 8, p593
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth.1354