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- Title
Agouti C57BL/6N embryonic stem cells for mouse genetic resources.
- Authors
Pettitt, Stephen J; Liang, Qi; Rairdan, Xin Y; Moran, Jennifer L; Prosser, Haydn M; Beier, David R; Lloyd, Kent C; Bradley, Allan; Skarnes, William C
- Abstract
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
- Publication
Nature methods, 2009, Vol 6, Issue 7, p493
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1342