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- Title
Rapid creation and quantitative monitoring of high coverage shRNA libraries.
- Authors
Bassik, Michael C; Lebbink, Robert Jan; Churchman, L Stirling; Ingolia, Nicholas T; Patena, Weronika; LeProust, Emily M; Schuldiner, Maya; Weissman, Jonathan S; McManus, Michael T
- Abstract
Short hairpin RNA libraries are limited by low efficacy of many shRNAs and by off-target effects, which give rise to false negatives and false positives, respectively. Here we present a strategy for rapidly creating expanded shRNA pools (approximately 30 shRNAs per gene) that are analyzed by deep sequencing (EXPAND). This approach enables identification of multiple effective target-specific shRNAs from a complex pool, allowing a rigorous statistical evaluation of true hits.
- Publication
Nature methods, 2009, Vol 6, Issue 6, p443
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1330