We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
- Authors
Nusbaum, Chad; Ohsumi, Toshiro K; Gomez, James; Aquadro, John; Victor, Thomas C; Warren, Robert M; Hung, Deborah T; Birren, Bruce W; Lander, Eric S; Jaffe, David B
- Abstract
Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives.
- Publication
Nature methods, 2009, Vol 6, Issue 1, p67
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1286