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- Title
Fluorescence nanoscopy by ground-state depletion and single-molecule return.
- Authors
Fölling, Jonas; Bossi, Mariano; Bock, Hannes; Medda, Rebecca; Wurm, Christian A; Hein, Birka; Jakobs, Stefan; Eggeling, Christian; Hell, Stefan W
- Abstract
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
- Publication
Nature methods, 2008, Vol 5, Issue 11, p943
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1257