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- Title
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.
- Authors
Hui-Wang Ai; Hazelwood, Kristin L.; Davidson, Michael W.; Campbell, Robert E.
- Abstract
Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET–based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.
- Publication
Nature Methods, 2008, Vol 5, Issue 5, p401
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth.1207