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- Title
Monovalent, reduced-size quantum dots for imaging receptors on living cells.
- Authors
Howarth, Mark; Liu, Wenhao; Puthenveetil, Sujiet; Zheng, Yi; Marshall, Lisa F; Schmidt, Michael M; Wittrup, K Dane; Bawendi, Moungi G; Ting, Alice Y
- Abstract
We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monovalency prevented EphA3 tyrosine kinase activation.
- Publication
Nature methods, 2008, Vol 5, Issue 5, p397
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1206