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- Title
Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.
- Authors
Taouatas, Nadia; Drugan, Madalina M; Heck, Albert J R; Mohammed, Shabaz
- Abstract
We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications.
- Publication
Nature methods, 2008, Vol 5, Issue 5, p405
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1204