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- Title
Identification of cross-linked peptides from large sequence databases.
- Authors
Rinner, Oliver; Seebacher, Jan; Walzthoeni, Thomas; Mueller, Lukas N; Beck, Martin; Schmidt, Alexander; Mueller, Markus; Aebersold, Ruedi
- Abstract
We describe a method to identify cross-linked peptides from complex samples and large protein sequence databases by combining isotopically tagged cross-linkers, chromatographic enrichment, targeted proteomics and a new search engine called xQuest. This software reduces the search space by an upstream candidate-peptide search before the recombination step. We showed that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search.
- Publication
Nature methods, 2008, Vol 5, Issue 4, p315
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1192