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- Title
Genetic control of neuronal activity in mice conditionally expressing TRPV1.
- Authors
Arenkiel, Benjamin R; Klein, Marguerita E; Davison, Ian G; Katz, Lawrence C; Ehlers, Michael D
- Abstract
Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.
- Publication
Nature methods, 2008, Vol 5, Issue 4, p299
- ISSN
1548-7105
- Publication type
Journal Article
- DOI
10.1038/nmeth.1190