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- Title
Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy.
- Authors
Pei-Lin Hsiung; Hardy, Jonathan; Friedland, Shai; Soetikno, Roy; Du, Christine B.; Wu, Amy P.; Sahbaie, Peyman; Crawford, James M.; Lowe, Anson W.; Contag, Christopher H0; Wang, Thomas D.
- Abstract
A combination of targeted probes and new imaging technologies provides a powerful set of tools with the potential to improve the early detection of cancer. To develop a probe for detecting colon cancer, we screened phage display peptide libraries against fresh human colonic adenomas for high-affinity ligands with preferential binding to premalignant tissue. We identified a specific heptapeptide sequence, VRPMPLQ, which we synthesized, conjugated with fluorescein and tested in patients undergoing colonoscopy. We imaged topically administered peptide using a fluorescence confocal microendoscope delivered through the instrument channel of a standard colonoscope. In vivo images were acquired at 12 frames per second with 50-μm working distance and 2.5-μm (transverse) and 20-μm (axial) resolution. The fluorescein-conjugated peptide bound more strongly to dysplastic colonocytes than to adjacent normal cells with 81% sensitivity and 82% specificity. This methodology represents a promising diagnostic imaging approach for the early detection of colorectal cancer and potentially of other epithelial malignancies.
- Publication
Nature Medicine, 2008, Vol 14, Issue 4, p454
- ISSN
1078-8956
- Publication type
Academic Journal
- DOI
10.1038/nm1692