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- Title
Humanized mice mount specific adaptive and innate immune responses to EBV and TSST-1.
- Authors
Melkus, Michael W; Estes, Jacob D; Padgett-Thomas, Angela; Gatlin, Joel; Denton, Paul W; Othieno, Florence A; Wege, Anja K; Haase, Ashley T; Garcia, J Victor
- Abstract
Here we show that transplantation of autologous human hematopoietic fetal liver CD34+ cells into NOD/SCID mice previously implanted with human fetal thymic and liver tissues results in long-term, systemic human T-cell homeostasis. In addition, these mice show systemic repopulation with human B cells, monocytes and macrophages, and dendritic cells (DCs). T cells in these mice generate human major histocompatibility complex class I- and class II-restricted adaptive immune responses to Epstein-Barr virus (EBV) infection and are activated by human DCs to mount a potent T-cell immune response to superantigens. Administration of the superantigen toxic shock syndrome toxin 1 (TSST-1) results in the specific systemic expansion of human Vbeta2+ T cells, release of human proinflammatory cytokines and localized, specific activation and maturation of human CD11c+ dendritic cells. This represents the first demonstration of long-term systemic human T-cell reconstitution in vivo allowing for the manifestation of the differential response by human DCs to TSST-1.
- Publication
Nature medicine, 2006, Vol 12, Issue 11, p1316
- ISSN
1078-8956
- Publication type
Journal Article
- DOI
10.1038/nm1431