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- Title
A role for LEDGF/p75 in targeting HIV DNA integration.
- Authors
Ciuffi, Angela; Llano, Manuel; Poeschla, Eric; Hoffmann, Christian; Leipzig, Jeremy; Shinn, Paul; Ecker, Joseph R; Bushman, Frederic
- Abstract
HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.
- Publication
Nature medicine, 2005, Vol 11, Issue 12, p1287
- ISSN
1078-8956
- Publication type
Journal Article
- DOI
10.1038/nm1329