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- Title
Signal amplification in molecular imaging by pretargeting a multivalent, bispecific antibody.
- Authors
Sharkey, Robert M; Cardillo, Thomas M; Rossi, Edmund A; Chang, Chien-Hsing; Karacay, Habibe; McBride, William J; Hansen, Hans J; Horak, Ivan D; Goldenberg, David M
- Abstract
Here we describe molecular imaging of cancer using signal amplification of a radiotracer in situ by pretargeting a multivalent, bispecific antibody to carcinoembryonic antigen (CEA), which subsequently also captures a radioactive hapten-peptide. Human colon cancer xenografts as small as approximately 0.15 g were disclosed in nude mice within 1 h of giving the radiotracer, with tumor/blood ratios increased by >or=40-fold (approximately 10:1 at 1 h, approximately 100:1 at 24 h), compared to a (99m)Tc-labeled CEA-specific F(ab') used clinically for colorectal cancer detection, while also increasing tumor uptake tenfold ( approximately 20% injected dose/g) under optimal conditions. This technology could be adapted to other antibodies and imaging modalities.
- Publication
Nature medicine, 2005, Vol 11, Issue 11, p1250
- ISSN
1078-8956
- Publication type
Journal Article
- DOI
10.1038/nm1322