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- Title
Steps toward mapping the human vasculature by phage display.
- Authors
Arap, Wadih; Kolonin, Mikhail G; Trepel, Martin; Lahdenranta, Johanna; Cardó-Vila, Marina; Giordano, Ricardo J; Mintz, Paul J; Ardelt, Peter U; Yao, Virginia J; Vidal, Claudia I; Chen, Limor; Flamm, Anne; Valtanen, Heli; Weavind, Lisa M; Hicks, Marshall E; Pollock, Raphael E; Botz, Gregory H; Bucana, Corazon D; Koivunen, Erkki; Cahill, Dolores; Troncoso, Patricia; Baggerly, Keith A; Pentz, Rebecca D; Do, Kim-Anh; Logothetis, Christopher J; Pasqualini, Renata
- Abstract
The molecular diversity of receptors in human blood vessels remains largely unexplored. We developed a selection method in which peptides that home to specific vascular beds are identified after administration of a peptide library. Here we report the first in vivo screening of a peptide library in a patient. We surveyed 47,160 motifs that localized to different organs. This large-scale screening indicates that the tissue distribution of circulating peptides is nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for differentially expressed cell-surface proteins, and a candidate ligand-receptor pair was validated. These data represent a step toward the construction of a molecular map of human vasculature and may have broad implications for the development of targeted therapies.
- Publication
Nature medicine, 2002, Vol 8, Issue 2, p121
- ISSN
1078-8956
- Publication type
Journal Article
- DOI
10.1038/nm0202-121