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- Title
Self-assembling nanocomplexes by combining ferumoxytol, heparin and protamine for cell tracking by magnetic resonance imaging.
- Authors
Thu, Mya S; Bryant, L Henry; Coppola, Tiziana; Jordan, E Kay; Budde, Matthew D; Lewis, Bobbi K; Chaudhry, Aneeka; Ren, Jiaqiang; Varma, Nadimpalli Ravi S; Arbab, Ali S; Frank, Joseph A
- Abstract
We report on a new straightforward magnetic cell-labeling approach that combines three US Food and Drug Administration (FDA)-approved drugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling nanocomplexes that effectively label cells for in vivo magnetic resonance imaging (MRI). We observed that the ferumoxytol-heparin-protamine (HPF) nanocomplexes were stable in serum-free cell culture medium. HPF nanocomplexes show a threefold increase in T2 relaxivity compared to ferumoxytol. Electron microscopy showed internalized HPF in endosomes, which we confirmed by Prussian blue staining of labeled cells. There was no long-term effect or toxicity on cellular physiology or function of HPF-labeled hematopoietic stem cells, bone marrow stromal cells, neural stem cells or T cells when compared to controls. In vivo MRI detected 1,000 HPF-labeled cells implanted in rat brains. This HPF labeling method should facilitate the monitoring by MRI of infused or implanted cells in clinical trials.
- Publication
Nature medicine, 2012, Vol 18, Issue 3, p463
- ISSN
1546-170X
- Publication type
Journal Article
- DOI
10.1038/nm.2666