We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels.
- Authors
Lecoq, Jérôme; Parpaleix, Alexandre; Roussakis, Emmanuel; Ducros, Mathieu; Goulam Houssen, Yannick; Vinogradov, Sergei A; Charpak, Serge
- Abstract
Uncovering principles that regulate energy metabolism in the brain requires mapping of partial pressure of oxygen (PO(2)) and blood flow with high spatial and temporal resolution. Using two-photon phosphorescence lifetime microscopy (2PLM) and the oxygen probe PtP-C343, we show that PO(2) can be accurately measured in the brain at depths up to 300 μm with micron-scale resolution. In addition, 2PLM allowed simultaneous measurements of blood flow and of PO(2) in capillaries with less than one-second temporal resolution. Using this approach, we detected erythrocyte-associated transients (EATs) in oxygen in the rat olfactory bulb and showed the existence of diffusion-based arterio-venous shunts. Sensory stimulation evoked functional hyperemia, accompanied by an increase in PO(2) in capillaries and by a biphasic PO(2) response in the neuropil, consisting of an 'initial dip' and a rebound. 2PLM of PO(2) opens new avenues for studies of brain metabolism and blood flow regulation.
- Publication
Nature medicine, 2011, Vol 17, Issue 7, p893
- ISSN
1546-170X
- Publication type
Journal Article
- DOI
10.1038/nm.2394