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- Title
In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response.
- Authors
Zhigang Fan; Spencer, Joel A.; Yan Lu; Pitsillides, Costas M.; Singh, Gurbakhshish; Kim, Pilhan; Yun, Seok H.; Toxavidis, Vasilis; Strom, Terry B.; Lin, Charles P.; Koulmanda, Maria
- Abstract
Here we present methods to longitudinally track islet allograft–infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (Teff cells) and discrimination between natural and induced regulatory T cells (nTreg and iTreg cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance.
- Publication
Nature Medicine, 2010, Vol 16, Issue 6, p718
- ISSN
1078-8956
- Publication type
Academic Journal
- DOI
10.1038/nm.2155