We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
AID mutant analyses indicate requirement for class-switch-specific cofactors.
- Authors
Van-Thanh Ta; Hitoshi Nagaoka; Nadia Catalan; Anne Durandy; Alain Fischer; Kohsuke Imai; Shigeaki Nonoyama; Junko Tashiro; Masaya Ikegawa; Satomi Ito; Kazuo Kinoshita; Masamichi Muramatsu; Tasuku Honjo
- Abstract
Activation-induced cytidine deaminase (AID) is the essential and sole B cell-specific factor required for class-switch recombination (CSR) and somatic hypermutation (SHM). However, it is not known how AID differentially regulates these two independent events. Involvement of several cofactors interacting with AID has been indicated by scattered distribution of loss-of-function point mutations and evolutionary conservation of the entire 198-amino-acid protein. Here, we report that human AID mutant proteins with insertions, replacements or truncations in the C-terminal region retained strong SHM activity but almost completely lost CSR activity. These results indicate that AID requires interaction with a cofactor(s) specific to CSR.
- Publication
Nature Immunology, 2003, Vol 4, Issue 9, p843
- ISSN
1529-2908
- Publication type
Academic Journal
- DOI
10.1038/ni964