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- Title
Regulation of thymocyte positive selection and motility by GIT2.
- Authors
Phee, Hyewon; Dzhagalov, Ivan; Mollenauer, Marianne; Wang, Yana; Irvine, Darrell J; Robey, Ellen; Weiss, Arthur
- Abstract
Thymocytes are highly motile cells that migrate under the influence of chemokines in distinct thymic compartments as they mature. The motility of thymocytes is tightly regulated; however, the molecular mechanisms that control thymocyte motility are not well understood. Here we report that G protein-coupled receptor kinase-interactor 2 (GIT2) was required for efficient positive selection. Notably, Git2(-/-) double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2(-/-) thymocytes was compromised in the thymic cortex, which suggests GIT2 has a key role in regulating the chemokine-mediated motility of double-positive thymocytes.
- Publication
Nature immunology, 2010, Vol 11, Issue 6, p503
- ISSN
1529-2916
- Publication type
Journal Article
- DOI
10.1038/ni.1868