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- Title
Genetic reconstruction of a functional transcriptional regulatory network.
- Authors
Zhanzhi Hu; Killion, Patrick J.; Iyer, Vishwanath R.
- Abstract
Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors.
- Publication
Nature Genetics, 2007, Vol 39, Issue 5, p683
- ISSN
1061-4036
- Publication type
Academic Journal
- DOI
10.1038/ng2012