We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis.
- Authors
Chen, Weisheng V; Delrow, Jeffrey; Corrin, Philip D; Frazier, Jason P; Soriano, Philippe
- Abstract
We developed a versatile, high-throughput genetic screening strategy by coupling gene mutagenesis and expression profiling technologies. Using a retroviral gene-trap vector optimized for efficient mutagenesis and cloning, we randomly disrupted genes in mouse embryonic stem (ES) cells and amplified them to construct a cDNA microarray. With this gene-trap array, we show that transcriptional target genes of platelet-derived growth factor (PDGF) can be efficiently and reliably identified in physiologically relevant cells and are immediately accessible to genetic studies to determine their in vivo roles and relative contributions to PDGF-regulated developmental processes. The same platform can be used to search for genes of specific biological relevance in a broad array of experimental settings, providing a fast track from gene identification to functional validation.
- Publication
Nature genetics, 2004, Vol 36, Issue 3, p304
- ISSN
1061-4036
- Publication type
Journal Article
- DOI
10.1038/ng1306