We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The developmental dynamics of the maize leaf transcriptome.
- Authors
Pinghua Li; Ponnala, Lalit; Gandotra, Neeru; Lin Wang; Yaqing Si; Tausta, S. Lori; Kebrom, Tesfamichael H.; Provart, Nicholas; Patel, Rohan; Myers, Christopher R.; Reidel, Edwin J.; Turgeon, Robert; Peng Liu; Qi Sun; Nelson, Timothy; Brutnell, Thomas P.
- Abstract
We have analyzed the maize leaf transcriptome using Illumina sequencing. We mapped more than 120 million reads to define gene structure and alternative splicing events and to quantify transcript abundance along a leaf developmental gradient and in mature bundle sheath and mesophyll cells. We detected differential mRNA processing events for most maize genes. We found that 64% and 21% of genes were differentially expressed along the developmental gradient and between bundle sheath and mesophyll cells, respectively. We implemented Gbrowse, an electronic fluorescent pictograph browser, and created a two-cell biochemical pathway viewer to visualize datasets. Cluster analysis of the data revealed a dynamic transcriptome, with transcripts for primary cell wall and basic cellular metabolism at the leaf base transitioning to transcripts for secondary cell wall biosynthesis and C4 photosynthetic development toward the tip. This dataset will serve as the foundation for a systems biology approach to the understanding of photosynthetic development.
- Publication
Nature Genetics, 2010, Vol 42, Issue 12, p1060
- ISSN
1061-4036
- Publication type
Academic Journal
- DOI
10.1038/ng.703