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- Title
Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks.
- Authors
Xionglei He; Wenfeng Qian; Zhi Wang; Ying Li; Jianzhi Zhang
- Abstract
Epistasis refers to the interaction between genes. Although high-throughput epistasis data from model organisms are being generated and used to construct genetic networks, the extent to which genetic epistasis reflects biologically meaningful interactions remains unclear. We have addressed this question through in silico mapping of positive and negative epistatic interactions amongst biochemical reactions within the metabolic networks of Escherichia coli and Saccharomyces cerevisiae using flux balance analysis. We found that negative epistasis occurs mainly between nonessential reactions with overlapping functions, whereas positive epistasis usually involves essential reactions, is highly abundant and, unexpectedly, often occurs between reactions without overlapping functions. We offer mechanistic explanations of these findings and experimentally validate them for 61 S. cerevisiae gene pairs.
- Publication
Nature Genetics, 2010, Vol 42, Issue 3, p272
- ISSN
1061-4036
- Publication type
Academic Journal
- DOI
10.1038/ng.524