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- Title
RNA polymerase V transcription guides ARGONAUTE4 to chromatin.
- Authors
Wierzbicki, Andrzej T; Ream, Thomas S; Haag, Jeremy R; Pikaard, Craig S
- Abstract
Retrotransposons and repetitive DNA elements in eukaryotes are silenced by small RNA-directed heterochromatin formation. In Arabidopsis, this process involves 24-nt siRNAs that bind to ARGONAUTE4 (AGO4) and facilitate the targeting of complementary loci via unknown mechanisms. Nuclear RNA polymerase V (Pol V) is an RNA silencing enzyme recently shown to generate noncoding transcripts at loci silenced by 24-nt siRNAs. We show that AGO4 physically interacts with these Pol V transcripts and is thereby recruited to the corresponding chromatin. We further show that DEFECTIVE IN MERISTEM SILENCING3 (DMS3), a structural maintenance of chromosomes (SMC) hinge-domain protein, functions in the assembly of Pol V transcription initiation or elongation complexes. Collectively, our data suggest that AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent Pol V transcripts.
- Publication
Nature genetics, 2009, Vol 41, Issue 5, p630
- ISSN
1546-1718
- Publication type
Journal Article
- DOI
10.1038/ng.365