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- Title
Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing.
- Authors
Qun Pan; Ofer Shai; Lee, Leo J.; Frey, Brendan J.; Blencowe, Benjamin J.
- Abstract
We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in ∼20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from ∼95% of multiexon genes undergo alternative splicing and that there are ∼100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.
- Publication
Nature Genetics, 2008, Vol 40, Issue 12, p1413
- ISSN
1061-4036
- Publication type
Academic Journal
- DOI
10.1038/ng.259