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- Title
Manipulating nucleosome disfavoring sequences allows fine-tune regulation of gene expression in yeast.
- Authors
Raveh-Sadka, Tali; Levo, Michal; Shabi, Uri; Shany, Boaz; Keren, Leeat; Lotan-Pompan, Maya; Zeevi, Danny; Sharon, Eilon; Weinberger, Adina; Segal, Eran
- Abstract
Understanding how precise control of gene expression is specified within regulatory DNA sequences is a key challenge with far-reaching implications. Many studies have focused on the regulatory role of transcription factor-binding sites. Here, we explore the transcriptional effects of different elements, nucleosome-disfavoring sequences and, specifically, poly(dA:dT) tracts that are highly prevalent in eukaryotic promoters. By measuring promoter activity for a large-scale promoter library, designed with systematic manipulations to the properties and spatial arrangement of poly(dA:dT) tracts, we show that these tracts significantly and causally affect transcription. We show that manipulating these elements offers a general genetic mechanism, applicable to promoters regulated by different transcription factors, for tuning expression in a predictable manner, with resolution that can be even finer than that attained by altering transcription factor sites. Overall, our results advance the understanding of the regulatory code and suggest a potential mechanism by which promoters yielding prespecified expression patterns can be designed.
- Publication
Nature genetics, 2012, Vol 44, Issue 7, p743
- ISSN
1546-1718
- Publication type
Journal Article
- DOI
10.1038/ng.2305