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- Title
Cu(I) recognition via cation-π and methionine interactions in CusF.
- Authors
Yi Xue; Davis, Anna V.; Balakrishnan, Gurusamy; Stasser, Jay P.; Staehlin, Benjamin M.; Focia, Pamela; Spiro, Thomas G.; Penner-Hahn, James E.; O'Halloran, Thomas V.
- Abstract
Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-π interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.
- Publication
Nature Chemical Biology, 2008, Vol 4, Issue 2, p107
- ISSN
1552-4450
- Publication type
Academic Journal
- DOI
10.1038/nchembio.2007.57