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- Title
ε-Tubulin is required for centriole duplication and microtubule organization.
- Authors
Chang, Paul; Giddings, Thomas H.; Winey, Mark; Stearns, Tim
- Abstract
Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified ε-tubulin as a new member of the tubulin superfamily that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of ε-tubulin to the new centrosome can only occur after exit from S phase and that ε-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis ε-tubulin was cloned and shown to be similar to human ε-tubulin in both sequence and localization. Depletion of ε-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that ε-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that ε-tubulin is required for centriole duplication and organization of the pericentriolar material.
- Publication
Nature Cell Biology, 2003, Vol 5, Issue 1, p71
- ISSN
1465-7392
- Publication type
Academic Journal
- DOI
10.1038/ncb900