We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Increased mobility of double-strand breaks requires Mec1, Rad9 and the homologous recombination machinery.
- Authors
Dion, Vincent; Kalck, Véronique; Horigome, Chihiro; Towbin, Benjamin D; Gasser, Susan M
- Abstract
Chromatin mobility is thought to facilitate homology search during homologous recombination and to shift damage either towards or away from specialized repair compartments. However, unconstrained mobility of double-strand breaks could also promote deleterious chromosomal translocations. Here we use live time-lapse fluorescence microscopy to track the mobility of damaged DNA in budding yeast. We found that a Rad52-YFP focus formed at an irreparable double-strand break moves in a larger subnuclear volume than the undamaged locus. In contrast, Rad52-YFP bound at damage arising from a protein-DNA adduct shows no increase in movement. Mutant analysis shows that enhanced double-strand-break mobility requires Rad51, the ATPase activity of Rad54, the ATR homologue Mec1 and the DNA-damage-response mediator Rad9. Consistent with a role for movement in the homology-search step of homologous recombination, we show that recombination intermediates take longer to form in cells lacking Rad9.
- Publication
Nature cell biology, 2012, Vol 14, Issue 5, p502
- ISSN
1476-4679
- Publication type
Journal Article
- DOI
10.1038/ncb2465