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- Title
Defining human ERAD networks through an integrative mapping strategy.
- Authors
Christianson, John C.; Olzmann, James A.; Shaler, Thomas A.; Sowa, Mathew E.; Bennett, Eric J.; Richter, Caleb M.; Tyler, Ryan E.; Greenblatt, Ethan J.; Wade Harper, J.; Kopito, Ron R.
- Abstract
Proteins that fail to correctly fold or assemble into oligomeric complexes in the endoplasmic reticulum (ER) are degraded by a ubiquitin- and proteasome-dependent process known as ER-associated degradation (ERAD). Although many individual components of the ERAD system have been identified, how these proteins are organized into a functional network that coordinates recognition, ubiquitylation and dislocation of substrates across the ER membrane is not well understood. We have investigated the functional organization of the mammalian ERAD system using a systems-level strategy that integrates proteomics, functional genomics and the transcriptional response to ER stress. This analysis supports an adaptive organization for the mammalian ERAD machinery and reveals a number of metazoan-specific genes not previously linked to ERAD.
- Publication
Nature Cell Biology, 2012, Vol 14, Issue 1, p93
- ISSN
1465-7392
- Publication type
Academic Journal
- DOI
10.1038/ncb2383