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- Title
Gene bookmarking accelerates the kinetics of post-mitotic transcriptional re-activation.
- Authors
Zhao, Rui; Nakamura, Tetsuya; Fu, Yu; Lazar, Zsolt; Spector, David L
- Abstract
Although transmission of the gene expression program from mother to daughter cells has been suggested to be mediated by gene bookmarking, the precise mechanism by which bookmarking mediates post-mitotic transcriptional re-activation has been unclear. Here, we used a real-time gene expression system to quantitatively demonstrate that transcriptional activation of the same genetic locus occurs with a significantly more rapid kinetics in post-mitotic cells versus interphase cells. RNA polymerase II large subunit (Pol II) and bromodomain protein 4 (BRD4) were recruited to the locus in a different sequential order on interphase initiation versus post-mitotic re-activation resulting from the recognition by BRD4 of increased levels of histone H4 Lys 5 acetylation (H4K5ac) on the previously activated locus. BRD4 accelerated the dynamics of messenger RNA synthesis by de-compacting chromatin and hence facilitating transcriptional re-activation. Using a real-time quantitative approach, we identified differences in the kinetics of transcriptional activation between interphase and post-mitotic cells that are mediated by a chromatin-based epigenetic mechanism.
- Publication
Nature cell biology, 2011, Vol 13, Issue 11, p1295
- ISSN
1476-4679
- Publication type
Journal Article
- DOI
10.1038/ncb2341